Thermo Fisher Scientific P-Selectin Polyclonal Antibody
P-selectin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(Paraffin)에 사용 가능. 인간과 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고순도(>95%) 제품. 연구용으로 세포 접착 및 염증 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human CD62P/P-selectin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803797 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein involved in regulating cell rolling. It is stored in the Weibel-Palade bodies of endothelial cells and in α-granules of platelets. Upon stimulation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or adenosine diphosphate, P-selectin is rapidly translocated to the cell surface.
It mediates interactions between activated endothelial cells or platelets and leukocytes, playing a key role in adhesive processes between leukocytes and endothelial cells. P-selectin is a calcium-dependent receptor that binds to sialylated Lewis blood group carbohydrate antigens on neutrophils and monocytes.
It is constitutively expressed during inflammation and contributes to atherogenesis, thrombosis, and tissue destruction. Clinically, P-selectin is used to distinguish heparin-induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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