Thermo Fisher Scientific BRSK1 Polyclonal Antibody
Thermo Fisher Scientific의 BRSK1 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. WB, IHC, ICC 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 고품질 제품입니다. 뇌 조직을 양성 대조로 권장하며, DNA 손상 체크포인트 연구에 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 28 amino acid peptide from near the center of human BRSK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155213 |
Product Specific Information
A suggested positive control is human brain tissue lysate.
PA5-20353 can be used with blocking peptide PEP-0473.
Target Information
BRSK1 (SAD1) is a serine/threonine kinase and a UV-induced DNA damage checkpoint kinase. BRSK1 is ubiquitously expressed, with highest levels in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression leads to G2/M arrest in HeLa S2 cells, and UV-induced G2/M arrest can be partially abrogated by reduced expression of BRSK1 through siRNA, indicating its role in DNA damage checkpoint function.
Both BRSK1 and BRSK2 are required for mammalian neuronal polarization. Double-mutant mice die within two hours of birth, and neurons from these mice show uniformly sized neurites displaying both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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