Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), NovaFluor Red 685, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 480 / 711 / 786
• FITC
• NovaFluor Red 700 / 755
• NovaFluor Yellow 700
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor that binds CCL19 and CCL21. CCR7 is expressed on T cells and dendritic cells (DCs), and plays a role in distinguishing naïve, central, and effector memory T cell populations. It is involved in T cell migration and DC maturation, chemotaxis, and survival.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for normal human peripheral blood cells. Use 5 µL (0.6 µg) per test in 100 µL final volume.
• Recommended incubation: ≥45 minutes at 2–8°C for optimal staining.
• Included reagents: 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, featuring engineered nucleic acid dye structures designed to reduce spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

CCR7 is a G-protein-coupled receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph nodes, and intestinal tissues. It regulates migration of memory T cells to inflamed tissues and promotes dendritic cell maturation. Ligands include chemokines CCL19 and CCL21. CCR family members (CCR1–CCR5) are key in T cell chemotaxis and phagocyte migration to inflammatory sites.

For Research Use Only. Not for use in diagnostic procedures.

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