Thermo Fisher Scientific HIF1AN Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor protein (2-254aa) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1AN,uniProtId:Q9NWT6-1,ncbiNodeId:9606,antigenRange:2-254,antigenLength:349,antigenImageFileName:PA5-117714_HIF1AN_Q9NWT6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117714_HIF1AN_Q9NWT6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2902343

Product Specific Information

This antibody has been tested in direct-ELISA

Target Information

Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.