Thermo Fisher Scientific HIF1AN Polyclonal Antibody
HIF1AN 단백질을 표적으로 하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot과 IHC(Paraffin) 실험에 적합하며, 인간 및 생쥐 시료에서 반응. Protein G로 정제된 비결합형 액상 항체로, 안정적인 저장 및 높은 특이성 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor protein (2–254aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2902343 |
Product Specific Information
This antibody has been tested in direct-ELISA.
Target Information
Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator.
Involved in transcriptional repression through interaction with HIF1A, VHL, and histone deacetylases.
Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A, and other ARD-containing proteins.
Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively.
Negatively regulates NOTCH1 activity, accelerating myogenic differentiation.
Positively regulates ASB4 activity, promoting vascular differentiation.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-117714_HIF1AN_Q9NWT6-1_Rabbit.svg, PA5-117714_HIF1AN_Q9NWT6-1_Rabbit_PDP.jpeg)
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