Thermo Fisher Scientific CDK2 Monoclonal Antibody (OTI1A8), TrueMAB
Human CDK2 단백질을 인식하는 Mouse Monoclonal Antibody로 Western Blot, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태, 1 mg/mL 농도, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A8 |
| Immunogen | Full length human recombinant protein of human CDK2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed to CyclinO. CyclinO is a specific protein isoform name for the UNG gene localized exclusively in the nucleus and exhibits uracil-DNA glycosylase activity.
CDK2/cyclinO protein levels increase during the G1 phase and are turned over during the cell cycle. Cyclin A-CDK2 levels peak at the G1/S transition and are associated with DNA during replication initiation. Rb protein acts as a substrate for CDK2-cyclin E and/or CDK2-cyclin A in vivo. CDK2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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