Thermo Fisher Scientific CD153 Chimeric Recombinant Rabbit Monoclonal Antibody (RM153)
Thermo Fisher Scientific의 CD153 키메릭 재조합 토끼 모노클로날 항체(RM153)는 마우스 CD153에 특이적으로 반응하며, ICC/IF, Flow Cytometry, Functional 및 Inhibition Assay에 사용 가능합니다. HEK293 발현 시스템에서 생산된 고순도 단백질 A 정제 항체로, 연구용으로 최적화되어 있습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Functional Assay (Functional) | Assay-dependent |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM153 |
| Immunogen | This antibody was raised by immunising Sprague Dawley rats with CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074609 |
Product Specific Information
This antibody is specific for murine CD153, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation and costimulation.
Target Information
The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin’s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection.
In addition to its expression on Hodgkin’s and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin’s lymphomas (including Burkitt’s lymphomas), virus-infected T and B cells, and on normal T and B cells after activation.
In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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