Thermo Fisher Scientific GPNMB Monoclonal Antibody (303822)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

8-25 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

303822

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Osteoactivin/GPNMBisoform 2 Ala22-Asn486 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GPNMB,uniProtId:Q14956-1,ncbiNodeId:9606,antigenRange:22-486,antigenLength:572,antigenImageFileName:MA5-24014_GPNMB_Q14956-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24014_GPNMB_Q14956-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607806

Product Specific Information

In direct ELISAs, no cross-reactivity with recombinant mouse Osteoactivin is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.