Thermo Fisher Scientific Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye

Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye

The Click-iT™ EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays, optimized for fluorescence microscopy applications. In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye through a fast and highly specific click reaction. This fluorescent labeling method provides accurate detection of proliferating cells and is compatible with antibody-based methods due to the mild click protocol.

주요 특징

• Simple: Works the first time, every time, in less time
• Efficient: No denaturation steps or harsh treatment required
• Content-rich results: Preserves cell morphology, antigen structure, and DNA integrity
• Consistent: Not dependent on variable antibody lots for detection

Superior Proliferation Methodology

The Click-iT™ EdU Cell Proliferation Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Mild fixation and detergent permeabilization allow the small molecule-based Click-iT™ detection reagent to access DNA easily. This results in an easy-to-use, accurate, and compatible assay for cell cycle analysis and other intracellular or extracellular targets, providing content-rich results.

This kit is optimized for fluorescence microscopy applications. For other platforms such as high-content imaging, fluorescence microplate, or flow cytometry, visit the Click-iT™ technology section on the Thermo Fisher website.

Notes

• The Click-iT™ assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods.
• It can be used with BrdU in dual pulse experiments using the anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.

사양 (Specifications)

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