Thermo Fisher Scientific Ly-49H Monoclonal Antibody (3D10), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer (NK) cells.
Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain, suggesting these molecules act as activation receptors.
Ly-49H is expressed in a subset of NK cells but not in NKT (NK1.1⁺CD3⁺) cells. NK cells can express several Ly-49 proteins.

Preferential staining of Ly-49H with Ly-49D has been observed (Product #00-5523-00).
For staining protocols, refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry.

Recommended usage: ≤0.125 µg per test (100 µL final volume).
Cell number per test: 10⁵–10⁸ cells (empirically determined).
Careful titration is recommended for optimal assay performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on NK cells.
Similar to Ly49D, it lacks ITAM and contains an arginine residue in the transmembrane domain, functioning as an activation receptor.
It is expressed in a subset of NK cells, but not in NKT (NK1.1/CD3⁺) cells. NK cells can express multiple Ly49 proteins.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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