Thermo Fisher Scientific MEK3 Polyclonal Antibody
Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합한 MEK3 폴리클로날 항체. 인간 유래 단백질에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며, -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human MAP2K3/MKK3 (UniProt ID: P46734-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2902651 |
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and is necessary for the expression of glucose transporter. It phosphorylates and activates MAPK14/p38-MAPK. This kinase can be activated by insulin and is required for glucose transporter expression. Expression of the RAS oncogene results in accumulation of the active form of this kinase, leading to constitutive activation of MAPK14 and oncogenic transformation of primary cells. Inhibition of this kinase is associated with the pathogenesis of Yersinia pseudotuberculosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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