Thermo Fisher Scientific IL1F9 Monoclonal Antibody (278706)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Neutralization (Neu)

0.15-0.6 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

278706

Immunogen

E. coli-derived recombinant human IL-36 gamma/IL-1F9 Met1-Asp169 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL1F9,uniProtId:Q9NZH8-1,ncbiNodeId:9606,antigenRange:1-169,antigenLength:169,antigenImageFileName:MA5-23907_IL1F9_Q9NZH8-1_Rat.svg,antigenImageFileNamePDP:MA5-23907_IL1F9_Q9NZH8-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576887

Product Specific Information

In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-36Ra/IL-1F5, rhIL-36 alpha /IL-1F6, rhIL-1F7, rhIL-36 beta /IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.