Thermo Fisher Scientific TGF beta-1 Monoclonal Antibody (TB21)
TGF beta-1 단백질을 인식하는 TB21 모노클론 항체로 Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 인간 및 다종 반응성. 비결합형 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Neutralization (Neu) | Assay-dependent | 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mustelid, Mink, Mouse, Sheep, Rabbit, Rat |
| Published Species | Guinea pig, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TB21 |
| Immunogen | TGF-beta 1 from human platelets |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction, Protein G |
| Storage Buffer | PBS, pH 7.0 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_561657 |
Product Specific Information
- Recommended positive controls: Ovine ovarian tissue, breast cancer sections
- Detects dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF beta under non-reducing and reducing conditions, respectively
- Store product as a concentrated solution and centrifuge briefly before opening the vial
Target Information
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found ubiquitously in tissues.
It is stored in the extracellular matrix as a latent complex with its pro-domain. Activation requires binding of αV integrin to an RGD sequence in the prodomain.
Latent forms are complexes of TGF beta-1, the latency-associated peptide (LAP), and latent TGF-beta binding proteins (LTBP).
TGF beta-1 regulates proliferation, differentiation, adhesion, and migration in various cell types. It can form homodimers or heterodimers with other TGF beta family members.
Mutations in TGFB1 are associated with Camurati-Engelmann disease and cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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