Thermo Fisher Scientific GRP78 Polyclonal Antibody
Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능한 GRP78 폴리클로날 항체. Rabbit IgG 유래로 항원 친화 크로마토그래피 정제. 인간 및 다종 반응성. 세포 스트레스 및 단백질 폴딩 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | View 1 publication |
| Flow Cytometry (Flow) | 1:150 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Sheep, Rat, Zebrafish |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal portion of the human GRP78 protein (within residues 250–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153375 |
Product Specific Information
This antibody is predicted to react with zebrafish, C. elegans, primate, bovine, and Drosophila based on 100% sequence homology.
Suggested positive control: HeLa whole cell extract.
Target Information
GRP78 is a 78 kDa glucose-regulated protein belonging to the HSP70 family. It resides in the endoplasmic reticulum (ER) and transiently associates with newly synthesized secretory and membrane proteins. GRP78 interacts with mutant or misfolded proteins, marking them for degradation.
The conserved sequence Lys-Asp-Glu-Leu (KDEL) at the C-terminus ensures ER retention via the KDEL receptor. GRP78 plays key roles in protein folding, degradation of misfolded proteins, and maintenance of cellular homeostasis.
It has been identified as a biomarker for hypoglycemia and exhibits neuroprotective effects against glutamate and oxidative stress. Reduced GRP78 expression is observed in Alzheimer’s disease and in association with mutations in the presenilin-1 (PS1) gene. Induction of GRP78 is also linked to drug resistance in cancer therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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