Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 480 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 660 / 755
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 645
• Request custom conjugation

Product Specific Information

The CD28.2 monoclonal antibody reacts with human CD28, a 44 kDa homodimer expressed on thymocytes, mature T cells, and plasma cells. CD28 acts as a ligand for CD80 (B7-1) and CD86 (B7-2), providing potent co-stimulation for T cell activation. Signaling through CD28 enhances IL-2 expression and cytotoxicity in CD3-activated T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended detection with a 610/20 bandpass filter. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57). Performance after fixation may vary by clone.

Excitation / Emission: 405 nm / 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD28 is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein of the immunoglobulin superfamily. It is a critical co-stimulatory receptor providing the second activation signal for T cells via binding to CD80 and CD86 on antigen-presenting cells. CD28 also prevents T cell anergy and premature apoptosis. In mice, CD28 is expressed on all CD4⁺ and CD8⁺ T cells, with expression varying by thymocyte maturation stage.
Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary’s disease.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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