Thermo Fisher Scientific CD169 Monoclonal Antibody (ED3)
Rat CD169 인식 단클론 항체(ED3)로 면역형광, 유세포분석, 면역조직화학 등 다양한 응용 가능. 단백질 A로 정제된 액상 항체로 0.5 mg/mL 농도. 비결합(Unconjugated) 형태이며 4°C 단기 보관, 장기 보관 시 -20°C 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:250 | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 2 publications |
| Flow Cytometry (Flow) | 1:500 | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ED3 |
| Immunogen | Rat spleen cell homogenate |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2189150 |
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-rat CD169 antibody, clone ED3, recognizes the rat CD169 cell surface antigen (~185 kDa) expressed by macrophages, predominantly confined to lymphoid organs.
Target Information
The siglecs are a family of membrane-bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific cell adhesion molecule expressed on activated macrophages in chronic inflammation and tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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