Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available on request

Product Specific Information

Description:
The eBio299Arm monoclonal antibody is used as an isotype control immunoglobulin.

Applications Reported:
Reported for use as an isotype control in flow cytometry.

Applications Tested:
Tested by LAL assay for endotoxin level and by immunofluorescent staining and flow cytometric analysis of mouse splenocytes and normal human peripheral blood.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody, determined by LAL assay.

Aggregation:
<10%, determined by HPLC.

Target Information

Isotype control antibodies serve as negative controls to distinguish non-specific background signals from specific antibody binding. They help account for background staining due to Fc receptor binding, non-specific interactions, or autofluorescence.
These controls are primarily used in flow cytometry but also applicable in ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the species and isotype of the primary antibody and, if directly labeled, use the same conjugate for accurate comparison.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.