Thermo Fisher Scientific V5 Tag Monoclonal Antibody (E10/V4RR), DyLight 650

Applications

Tested Dilution

Publications

Western Blot (WB)

-

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Immunocytochemistry (ICC/IF)

1:25-1:100

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Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E10/V4RR

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

View additional formats

• Unconjugated
• Biotin
• DyLight 488
• DyLight 680
• HRP
• Request custom conjugation

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537642

Product Specific Information

MA5-15253-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.