Thermo Fisher Scientific POU2AF1 Monoclonal Antibody (UMAB219), UltraMAB
POU2AF1 단백질을 인식하는 Mouse monoclonal 항체로, IHC, ChIP, Peptide Array 등 다양한 응용에 적합합니다. Human 시료에 반응하며, Lyophilized 형태로 제공됩니다. 고순도의 Affinity chromatography 정제 제품으로 연구용에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:250 |
| ChIP assay (ChIP) | Assay-Dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB219 |
| Immunogen | Full length human recombinant protein of human POU2AF1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
POU2AF1 is a transcriptional coactivator that specifically associates with OCT1 or OCT2, enhancing OCT1-mediated promoter activity and to a lesser extent that of OCT2.
It lacks intrinsic DNA-binding activity but recognizes the POU domains of OCT1 and OCT2.
This protein is essential for B-cell antigen response and germinal center formation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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