Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody (NSE-P2)
Neuron Specific Enolase(NSE) γ 단백질을 인식하는 마우스 단일클론 항체로, 신경 및 신경내분비 세포 표지에 적합. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 고순도 Protein A/G 정제, 200 µg/mL 농도, 안정적인 PBS 버퍼 포뮬레이션.
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- 2026-MSM5-P0
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Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody (NSE-P2)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NSE-P2 |
| Immunogen | Synthetic peptide of human NSE gamma (around aa416–433) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse in gluconeogenesis.
NSE is stable in biological fluids and diffuses into extracellular medium and CSF upon neuronal injury.
It is one of three mammalian enolases (α, β, γ), expressed respectively in most tissues, muscle, and neurons/neuroendocrine cells.
Co-expression with chromogranin A is common in neuroendocrine neoplasms.
NSE antibodies are valuable for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells.
Elevated NSE levels serve as biomarkers for neuronal injury and are clinically used in diagnosing stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, herpetic encephalitis, and certain cancers such as neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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