Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.1 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780
• Brilliant UV/Violet/Yellow series, eFluor 450, FITC, PE, PE-Cyanine5.5, PE-Cyanine7, PerCP-eFluor 710
• NovaFluor Blue/Red/Violet/Yellow variants
• Super Bright series
• Custom conjugation available upon request

Product Specific Information

Description:
The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by most thymocytes, a subset of mature T helper cells, and at low levels by monocytes. CD4 serves as a receptor for HIV. The SK3 clone blocks HIV binding and mixed lymphocyte reaction. SK3 and RPA-T4 recognize distinct epitopes.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested for flow cytometry on normal human peripheral blood cells
• Recommended use: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL buffer per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates use Phiton™ technology, enabling engineered fluorescent signatures with reduced spillover and spread.

Optical Properties:

• Excitation: 494 nm
• Emission: 585 nm
• Laser: 488 nm (Blue)

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T helper cells, and also found on monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. CD4 participates in early T-cell activation through association with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.