Thermo Fisher Scientific CD83 Recombinant Rabbit Monoclonal Antibody (017)

Applications

ELISA (ELISA)

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the rabbit immune repertoire

This antibody has specificity for Human CD83.

Target Information

CD83 is a 40–45 kDa cell surface glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 after stimulation with agents such as Con A or PHA. In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by cells within germinal centers. It is also expressed by Langerhans cells in the skin.

The CD83 antigen is a 186-amino-acid single-chain glycoprotein, a member of the immunoglobulin superfamily, containing:

• An extracellular V-type Ig-like domain
• A transmembrane region
• A short cytoplasmic tail (~40 amino acids)

CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of the core protein. CD83⁺ cells exhibit a unique surface phenotype distinct from T, B, NK, or myelomonocytic cells, and co-express high levels of MHC class II molecules along with various lineage markers (CD2, CD5, CD40, CD78, CD13, CD33, CD36).

Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.