Thermo Fisher Scientific Human Furin Recombinant Protein

Applications

Western Blot (WB)

• Assay-dependent
  View 2 publications

Neutralization (Neu)

• Not applicable
  View 1 publication

Control (Ctrl)

• Assay-dependent
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Miscellaneous PubMed (Misc)

• Not applicable
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Product Specifications

Product Specific Information

RP-062 has an activity of 1 U/µL, where one unit (1 U) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in one minute.
As a fully functional enzyme, RP-062 can be used in various enzymatic assays.
This furin construct represents amino acid residues 1–713 from human furin convertase protein.

Target Information

Furin is a membrane-associated, calcium-dependent serine protease belonging to the subtilisin-like prohormone convertase (PC) family. These enzymes cleave most prohormones and neuropeptide precursors, as well as numerous cellular, viral, and bacterial proteins transported via the secretory pathway.

Furin and other PC family members share structural features:

• ~10 kDa amino-terminal proregion
• ~55 kDa conserved subtilisin-like catalytic domain
• Variable carboxyl-terminal domain

Activation occurs following proregion cleavage in the appropriate cellular compartment.
Furin is the only known PC possessing a transmembrane domain.
Cleavage of target proteins occurs at the carboxyl-terminus of the consensus sequence RX(K/R)R.
The acidic peptide sequence C771PSDSEEDEG780 localizes furin to the trans-Golgi network, and phosphorylation of serine residues in this region modulates intracellular routing.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.