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Thermo Fisher Scientific Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2, 6418)
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Bcl-2 단백질을 인식하는 마우스 유래 재조합 단일클론 항체. Western blot, IHC, ICC, Flow cytometry에 적합. HEK293 세포에서 발현, Protein A/G로 정제. 항세포사멸 단백질 연구에 활용 가능.
- 카탈로그번호
- 596-MSM14-P1
- 판매단위
- pk
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마지막 업데이트 2025. 08. 02. 오후 06:59
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Thermo Fisher Scientific 596-MSM14-P1 Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2, 6418) 100 ug pk판매 단위 pk ·
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829,000원VAT 포함 911,900원
Thermo Fisher Scientific · Thermo Fisher Scientific Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2, 6418)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rBCL2, 6418 |
| Immunogen | Synthetic peptide, aa41–54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed), incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BCL-2 is a key regulator of apoptosis that can inhibit or promote cell death. Members of the BCL-2 family form dimers to modulate apoptosis.
- Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect.
- Bax and Bak promote cytochrome c release from mitochondria, initiating apoptosis.
- Overexpression or translocation of BCL2 is linked to follicular lymphoma and other cancers such as B-cell lymphoma and prostate cancer.
Two transcript variants of BCL-2 differ in their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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