Thermo Fisher Scientific Phosphothreonine Polyclonal Antibody
Western blot과 IHC(P)에서 사용 가능한 폴리클로날 항체로, phosphothreonine 인산화 단백질 검출에 적합. Rabbit IgG 기반, 항원 친화 정제 방식으로 제조. 안정화제가 포함된 PBS 용액 형태로 제공되며, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A chemically linked phosphothreonine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: A431 (EGF stimulated)
Target Information
Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine.
There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine.
Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase.
Small amounts of free phosphothreonine can be detected in urine.
Antibodies against phosphothreonine can be used as identifiers in the analysis of peptides, proteins, and enzymes.
[Source: National Center for Biotechnology Information]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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