Thermo Fisher Scientific RAB3D Monoclonal Antibody (5E9), APC
RAB3D 단백질을 검출하기 위한 APC 결합 단일클론 항체로, Western blot에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 단백질 G로 정제된 고순도 항체입니다. 형광 특성은 651/660 nm이며, 보존제가 포함되어 있지 않습니다.
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Applications
- Western Blot (WB): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E9 |
| Immunogen | Full length recombinant RAB3D protein |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932423 |
Additional Formats
- FITC (Cat. No. MA5-45970)
- PerCP (Cat. No. MA5-45971)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45969 was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, and kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 24 kDa. Does not show cross-reactivity to RAB3A, RAB3B, or RAB3C.
Target Information
Rab proteins are low-molecular-weight GTP-binding proteins belonging to the Ras superfamily of GTPases. They are localized on the cytoplasmic face of organelles and vesicles and are involved in intracellular membrane fusion reactions. Core fusion machinery includes SNAP-25, synaptobrevin, and syntaxin, which interact with NSF and α-SNAP. Rab proteins, with Munc-18, mediate vesicle docking and fusion. Over 40 Rab proteins have been described in mammals. Rab3D is expressed in tissues such as tongue, pancreas, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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