Thermo Fisher Scientific Decorin Monoclonal Antibody (DCN, 3523)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Peptide Array (Array)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

DCN, 3523

Immunogen

Recombinant human Decorin protein fragment (aa212-336) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Decorin,uniProtId:P07585-1,ncbiNodeId:9606,antigenRange:212-336,antigenLength:359,antigenImageFileName:1634-MSM3-P0_Decorin_P07585-1_House_mouse.svg,antigenImageFileNamePDP:1634-MSM3-P0_Decorin_P07585-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-b. It is believed that decorin also plays a role in the cell cycle of chondrocytes. The decorin neoepitope fragment has a sequence of PKTLQE, and is generated by MMP-3 or ADAMTS-4 cleavage at Glu154-Leu155 of decorin core protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.