Thermo Fisher Scientific CD57 Monoclonal Antibody (TB01 (TBO1)), eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 12 publications 12 publications

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgM

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TB01 (TBO1)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• Unconjugated
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2016613

Product Specific Information

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.