Thermo Fisher Scientific NOX3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

15 amino acid peptide near the amino terminus of human NOX3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NOX3,uniProtId:Q9HBY0-1,ncbiNodeId:9606,antigenRange:1,antigenLength:568,antigenImageFileName:PA5-38036_NOX3_Q9HBY0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-38036_NOX3_Q9HBY0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554640

Product Specific Information

PA5-38036 detects Nox3 in mouse bone marrow and humanG-CSF mobilized CD34+ cells in immunofluorescence analysis.

A recommended positive control is 293 cell lysate.

NOX3 is not expected to cross-react with other NOX proteins.

Target Information

The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide. NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith. It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.