Thermo Fisher Scientific ADAR1 Polyclonal Antibody
ADAR1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합합니다. 인간 ADAR1의 950–1000번 영역을 면역원으로 사용하였으며, 4°C 보관 시 1년 안정적입니다. Mouse 및 Rat에도 반응 예측됩니다.
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Thermo Fisher Scientific ADAR1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 950 and 1000 of human Adenosine Deaminase, RNA-specific (ADAR1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), referred to as A-to-I RNA editing. This process can affect gene expression and function by altering codons, splice sites, RNA stability, and RNA structure-dependent activities such as microRNA production and protein-RNA interactions.
ADAR can edit both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing). Its cellular RNA substrates include:
- Bladder cancer-associated protein (BLCAP)
- Neurotransmitter receptors for glutamate (GRIA2), serotonin (HTR2C), and GABA receptor (GABRA3)
Site-specific RNA editing of these transcripts leads to amino acid substitutions that alter protein function.
Viral RNA substrates include:
- Hepatitis C virus (HCV)
- Vesicular stomatitis virus (VSV)
- Measles virus (MV)
- Hepatitis delta virus (HDV)
- Human immunodeficiency virus type 1 (HIV-1)
ADAR1 exhibits proviral or antiviral effects depending on the virus and mechanism:
- Proviral: HDV, MV, VSV, HIV-1
- Antiviral: HCV
It can modulate viral replication via editing-dependent or editing-independent mechanisms. For example:
- Inhibits HCV replication through RNA editing
- Enhances MV, VSV, and HIV-1 replication by suppressing EIF2AK2/PKR activation
- Stimulates HIV-1 particle release and infectivity through editing in the 5′UTR and coding sequences of Rev and Tat
- Enhances HDV replication via A-to-I editing that converts a stop codon (amber) to a tryptophan codon, allowing synthesis of the large delta antigen (L-HDAg) required for viral assembly
- High ADAR1 expression can inhibit HDV replication
(Source: UniProt)
Additional Information
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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