Thermo Fisher Scientific ADAR1 Polyclonal Antibody

Thermo Fisher Scientific ADAR1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), referred to as A-to-I RNA editing. This process can affect gene expression and function by altering codons, splice sites, RNA stability, and RNA structure-dependent activities such as microRNA production and protein-RNA interactions.

ADAR can edit both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing). Its cellular RNA substrates include:

• Bladder cancer-associated protein (BLCAP)
• Neurotransmitter receptors for glutamate (GRIA2), serotonin (HTR2C), and GABA receptor (GABRA3)

Site-specific RNA editing of these transcripts leads to amino acid substitutions that alter protein function.

Viral RNA substrates include:

• Hepatitis C virus (HCV)
• Vesicular stomatitis virus (VSV)
• Measles virus (MV)
• Hepatitis delta virus (HDV)
• Human immunodeficiency virus type 1 (HIV-1)

ADAR1 exhibits proviral or antiviral effects depending on the virus and mechanism:

• Proviral: HDV, MV, VSV, HIV-1
• Antiviral: HCV

It can modulate viral replication via editing-dependent or editing-independent mechanisms. For example:

• Inhibits HCV replication through RNA editing
• Enhances MV, VSV, and HIV-1 replication by suppressing EIF2AK2/PKR activation
• Stimulates HIV-1 particle release and infectivity through editing in the 5′UTR and coding sequences of Rev and Tat
• Enhances HDV replication via A-to-I editing that converts a stop codon (amber) to a tryptophan codon, allowing synthesis of the large delta antigen (L-HDAg) required for viral assembly
• High ADAR1 expression can inhibit HDV replication

(Source: UniProt)

Additional Information

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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