Thermo Fisher Scientific ADAR1 Polyclonal Antibody

Thermo Fisher Scientific ADAR1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), referred to as A-to-I RNA editing.
This process can affect gene expression and function by:

• Modifying codons and amino acid sequences in proteins
• Altering splice site recognition sequences in pre-mRNA
• Changing RNA stability and nuclease recognition
• Influencing RNA virus genome replication
• Regulating microRNA production and RNA-protein interactions

ADAR can edit both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing).
Its cellular RNA substrates include:

• BLCAP (bladder cancer-associated protein)
• GRIA2 (glutamate receptor)
• HTR2C (serotonin receptor)
• GABRA3 (GABA receptor)

Viral RNA substrates include:

• Hepatitis C virus (HCV)
• Vesicular stomatitis virus (VSV)
• Measles virus (MV)
• Hepatitis delta virus (HDV)
• HIV-1

ADAR1 exhibits both proviral and antiviral effects, depending on virus type and mechanism (editing-dependent or independent).
It can impair HCV replication via RNA editing and enhance replication of MV, VSV, and HIV-1 through suppression of EIF2AK2/PKR activation.
In HDV, ADAR1 promotes viral replication via A-to-I editing at the amber/W site, converting a stop codon to a tryptophan codon, allowing synthesis of the large delta antigen (L-HDAg).
However, high levels of ADAR1 inhibit HDV replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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