Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 인간 시료 반응성이 있으며 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TRIM25 (Product #RP-89334) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2856112 |
Product Specific Information
Immunogen sequence:
FCPEHSECIC HICLVEHKTC SPASLSQASA DLEATLRHKL TVMYSQINGA SRALDDVRNR QQDVRMTANR KVEQLQQEYT EMKALLDASE TTSTRKIKEE EKRVNSKFDT IYQILLKKKS EIQTL
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α.
Mice lacking this gene exhibit an underdeveloped uterus and reduced estrogen responsiveness, indicating its essential role in estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, promoting tumor growth via E3 ubiquitin ligase activity that targets the negative cell cycle checkpoint 14-3-3σ.
It is also crucial for the RIG-1 signaling pathway in antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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