Thermo Fisher Scientific Claudin 1 Polyclonal Antibody (MH25)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 72 publications 72 publications

Immunohistochemistry (IHC)

-

View 41 publications 41 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

-

View 44 publications 44 publications

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

-

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Avian, Bovine, Chicken, Dog, Fish, Human, Mouse, Pig, Rabbit, Rat, Tag, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

MH25

Immunogen

A synthetic peptide derived from the C-terminus of the human/mouse Claudin-1 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 1,uniProtId:O95832-1,ncbiNodeId:9606,antigenRange:211,antigenLength:211,antigenImageFileName:71-7800_Claudin_1_O95832-1_Rabbit.svg,antigenImageFileNamePDP:71-7800_Claudin_1_O95832-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533997

Product Specific Information

This antibody reacts with the ~ 22 kDa Claudin-1 protein. Reactivity was confirmed by western blotting and immunofluorescence. Positive controls include MDCK cells (dog), Caco-2cells (human), mouse liver, rat liver, and rat kidney.

NOTE: This antibody strongly cross reacts with the Claudin-3 protein. It has been observed in western blotting inhibition studies using Caco-2 cell lysates, that this antibody is inhibited by addition of Claudin-3 peptide. For a Claudin-1 specific antibody that does not cross-react with Claudin-3, please order Cat. No. 519000 (Rb x Claudin-1) instead.

Target Information

Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.