Thermo Fisher Scientific DNA Ligase IV Recombinant Rabbit Monoclonal Antibody (ARC0595)
Recombinant Rabbit Monoclonal Antibody for DNA Ligase IV (ARC0595). Human 반응성, Western blot 및 ELISA에 적합. 고순도 Affinity Chromatography 정제, PBS/glycerol buffer 보존. -20°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0595 |
| Immunogen | Recombinant fusion protein containing amino acids 504–685 of human DNA Ligase IV (P49917) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.47 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849014 |
Product Specific Information
Immunogen sequence:
SRVGSGCTMK ELYDLGLKLA KYWKPFHRKA PPSSILCGTE KPEVYIEPCN SVIVQIKAAE IVPSDMYKTG CTLRFPRIEK IRDDKEWHEC MTLDDLEQLR GKASGKLASK HLYIGGDDEP QEKKRKAAPK MKKVIGIIEH LKAPNLTNVN KISNIFEDVE FCVMSGTDSQ PKPDLENRIA EF
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). It forms a complex with XRCC4 and interacts with DNA-PK, both required for NHEJ. The crystal structure of the XRCC4–DNA Ligase IV complex has been resolved. Defects in this gene cause LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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