Thermo Fisher Scientific ABCA1 Monoclonal Antibody (HJ1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

1 µg per million cells

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

HJ1

Immunogen

50 kDa N-terminal extracellular loop of ABCA1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157018

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Target Information

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.