Thermo Fisher Scientific ABCA1 Monoclonal Antibody (HJ1)
ABCA1 단백질을 인식하는 HJ1 클론 단일클론 항체로, 인간·마우스·랫트 반응성이 있음. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 단백질 G 정제, 1 mg/mL 농도, 비결합형 액상 제품. 콜레스테롤 수송 관련 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1 µg per million cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HJ1 |
| Immunogen | 50 kDa N-terminal extracellular loop of ABCA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157018 |
Product Specific Information
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Target Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by absence of plasma HDL, cholesterol ester deposition in the reticulo-endothelial system, and deviations in cellular lipid trafficking.
ABCA1 mediates apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and Golgi complex, and is regulated by cholesterol flux.
Regulation of cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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