Thermo Fisher Scientific MMTV Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

ELISA (ELISA)

1:10,000-1:20,000

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

66 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis was used to suggest cross-reactivity with MMTV from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Target Information

Mouse mammary tumor virus capsid is a polyprotein that is cleaved by aspartyl protease during or after the release of the virion from the plasma membrane. After entering the cell, reverse transcriptase converts the viral dimeric RNA genome into dsDNA in the cytoplasm. Displaying DNA polymerase activity and RNase H activity, this enzyme copies either DNA or RNA templates and cleaves the RNA strand of the RNA-DNA heteroduplex in a partially processive 3to 5 endonucleasic mode. tRNS binds to the primer at the 5end of the viral RNA. Reverse transcriptase uses the 3 end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis proceeding through the U5 region and ending after the repeated region. This RNA-DNA heteroduplex is digested by the RNase H and hybridizes with the identical R region at the 3end of the viral RNA. RNase H then digests the RNA template except for a polypurine tract situated at the 5 end of the genome. RNase H probably can proceed both in a polymerase-dependent and a polymerase-independent mode. Reverse transcriptase also performs DNA-directed plus-strand DNA synthesis using the polypurine tract that has not been removed by RNase H as primers. Polypurine tract and tRNA primers are then removed by RNase H and the 3and 5 ssDNA primer binding site regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by reverse transcriptase to the primer binding site and polypurine tract ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats at both ends. Anti-MMTV Antibody is ideal for researchers interested in Cancer, Enzyme, and Viral research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.