Thermo Fisher Scientific MMTV Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

A BLAST analysis suggests cross-reactivity with MMTV from mouse based on 100% homology with the immunizing sequence. Reactivity with homologues from other species is not known.

Target Information

Mouse mammary tumor virus (MMTV) capsid is a polyprotein cleaved by aspartyl protease during or after virion release from the plasma membrane. Upon cell entry, reverse transcriptase converts the viral RNA genome into double-stranded DNA (dsDNA) in the cytoplasm. The enzyme exhibits both DNA polymerase and RNase H activities, enabling it to copy DNA or RNA templates and cleave RNA strands of RNA-DNA hybrids.

tRNA binds to the primer site at the 5′ end of viral RNA, initiating minus-strand DNA synthesis through the U5 region. RNase H digests RNA templates except for the polypurine tract, which serves as a primer for plus-strand synthesis. Subsequent removal of primers and hybridization of complementary regions form a circular dsDNA intermediate. Strand displacement synthesis produces a linear dsDNA copy of the viral genome with long terminal repeats at both ends.

Anti-MMTV Antibody is ideal for researchers engaged in cancer, enzyme, and viral research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.