Thermo Fisher Scientific FZD5 Polyclonal Antibody
Rabbit polyclonal antibody targeting human FZD5. Validated for IHC(P) and ELISA. High purity via antigen affinity chromatography. Suitable for multiple species including human, dog, and rabbit. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 7 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Non-human primate, Pig, Rabbit |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD5 / Frizzled 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550232 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bovine, Elephant, Rabbit, Pig (100%)
- Mouse, Rat, Hamster (93%)
- Opossum (86%)
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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