Thermo Fisher Scientific Biotin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:5,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

In Situ Hybridization (ISH)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Published species

Bacteria, Chemical, Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Biotin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 20mg/mL sucrose

Contains

0.08% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228243

Product Specific Information

The activity measured by a solid phase binding assay is 99%.

Reconstitute using 1 mL of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.