Thermo Fisher Scientific F(ab`)2-Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, PE

Applications

Tested Dilution

Flow Cytometry (Flow)

1:50 - 1:200

Product Specifications

Species Reactivity

Goat

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

PE PE PE

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Donkey/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Donkey/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Donkey/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Donkey/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Donkey/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Donkey/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Donkey/)
• [AP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [DSB-X Biotin](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DSB-X Biotin/host/Donkey/)
• [DyLight 350](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Donkey/)
• [DyLight 488](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Donkey/)
• [DyLight 550](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Donkey/)
• [DyLight 594](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Donkey/)
• [DyLight 650](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Donkey/)
• [DyLight 680](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Donkey/)
• [DyLight 755](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Donkey/)
• [DyLight 800](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Goat IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient

RRID

AB_429714

Target

IgG

Cross Adsorption

Against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31860 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31860 reacts with the heavy chains of goat IgG and with light chains common to most goat immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.

Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 82.0 at 566 nm for a

1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)

Phycoerythrin Source: Seaweed

Store product protected from light at 4°C until opened. DO NOT FREEZE. RPE Amax= 490 nm, 545 nm, and 566 nm; Emax= 580 nm.

Reconstitute with 1.0 mL of distilled water (0.5 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.