Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Super Bright 645, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 702, 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.

Applications Reported:
This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA.

Applications Tested:
Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.

Fluorescence Properties:

• Excitation: 405 nm
• Emission: 645 nm
• Laser: Violet Laser

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants.

Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also employed in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens.

Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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