Thermo Fisher Scientific 6x His Tag Chimeric Recombinant Rat Monoclonal Antibody (3D5)
6x His 태그 단백질 검출용 랫트 모노클로날 항체. C-말단 및 내부 His-tag에 특이적으로 결합. Western blot 및 ELISA에 사용 가능. 고순도 Protein A 정제, 액상 형태로 제공. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rat / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | Purified His-tagged Fab fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074199 |
Product Specific Information
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins using known epitopes when no specific antibody is available. His-tagged proteins can be purified efficiently due to the strong and selective chelation of histidine residues to Ni²⁺ ions on Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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