Thermo Fisher Scientific DGKA Monoclonal Antibody (OTI4A11), TrueMAB
DGKA 단백질을 인식하는 Mouse IgG2b 형 단일클론 항체로, Western blot, IHC, Flow Cytometry에 사용 가능. HEK293T 유래 전장 인간 DGKA 항원을 면역원으로 제작. 고순도 친화 크로마토그래피 정제, 무보존제, 동결건조 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A11 |
| Immunogen | Full-length human recombinant DGKA protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
DGKA encodes a member of the eukaryotic diacylglycerol kinase family. It modulates intracellular signaling by competing with protein kinase C for diacylglycerol, contributes to phosphatidylinositol resynthesis, and phosphorylates diacylglycerol to phosphatidic acid.
Alternative splicing produces four transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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