Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• FITC
• NovaFluor Blue Series (510, 530, 555, 585, 610-30S, 610-70S, 660-40S, 660-120S, 725, 760, 800)
• NovaFluor Red Series (660, 685, 700, 710, 725, 755)
• NovaFluor Violet Series (690, 745, 800)
• NovaFluor Yellow Series (570, 590, 610, 660, 690, 700, 730, 755, 810)
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Request custom conjugation available

Product Specific Information

The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, T helper cells, and low levels on monocytes. CD4 acts as a receptor for HIV, and SK3 blocks HIV binding and mixed lymphocyte reaction. SK3 and RPA-T4 antibodies recognize distinct epitopes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides improved formulation over previous versions. Add 5 µL per sample for optimal staining in antibody cocktails or single-color controls.

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for minimal spillover and spread effects.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD4 antigen is involved in MHC class II molecule recognition and serves as a co-receptor for HIV. It is primarily expressed in T helper cells, as well as monocytes, macrophages, and dendritic cells. CD4 is found in thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. It plays a role in early T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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