Thermo Fisher Scientific HSPA4 Recombinant Rabbit Monoclonal Antibody (ARC2237)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2237

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human HSPA4 (P34932) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSPA4,uniProtId:P34932-1,ncbiNodeId:9606,antigenRange:700-800,antigenLength:840,antigenImageFileName:MA5-37957_HSPA4_P34932-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37957_HSPA4_P34932-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.18 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897877

Product Specific Information

Positive Samples: HeLa, 293T, NIH/3T3, K-562, C6, Mouse testis, Mouse brain, Rat brain

Immunogen sequence: EELGKQIQQY MKIISSFKNK EDQYDHLDAA DMTKVEKSTN EAMEWMNNKL NLQNKQSLTM DPVVKSKEIE AKIKELTSTC SPIISKPKPK VEPPKEEQKN A

Target Information

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.