Thermo Fisher Scientific GFP Polyclonal Antibody, Rhodamine

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse, and Rat Serum Proteins.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP enables localization studies of proteins, tracking their movement, and exploring subcellular compartment dynamics. GFP technology has provided new insights into cellular physiology. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm) without requiring additional cofactors. Its fluorescence is stable, species-independent, and suitable for a variety of applications including gene expression monitoring, protein localization, protein–protein interaction assays (yeast two-hybrid), and FRET-based distance measurements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지