Thermo Fisher Scientific Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody (2M1K10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2M1K10

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_0002141) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 12,uniProtId:Q99456-1,ncbiNodeId:9606,antigenRange:250-494,antigenLength:494,antigenImageFileName:MA5-42701_Cytokeratin_12_Q99456-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42701_Cytokeratin_12_Q99456-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911842

Product Specific Information

Positive test controls include: Rat eye.

Immunogen sequence: EMQIESLNEE LAYMKKNHED ELQSFRVGGP GEVSVEMDAA PGVDLTRLLN DMRAQYETIA EQNRKDAEAW FIEKSGELRK EISTNTEQLQ SSKSEVTDLR RAFQNLEIEL QSQLAMKKSL EDSLAEAEGD YCAQLSQVQQ LISNLEAQLL QVRADAERQN VDHQRLLNVK ARLELEIETY RRLLDGEAQG DGLEESLFVT DSKSQAQSTD SSKDPTKTRK IKTVVQEMVN GEVVSSQVQE IEELM

Target Information

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.