Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Species Reactivity

• Chimpanzee
• Human

Host / Isotype

• Mouse / IgG1, kappa

Class

• Monoclonal

Type

• Antibody

Clone

• SK7

Conjugate

• Brilliant Ultra Violet™ 661

Available Formats:

• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Custom conjugation available

Excitation / Emission Max

• 349 / 659 nm

Product Specifications

Product Specific Information

The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells
• Recommended Usage: 5 µL (1.0 µg)/test in a final volume of 100 µL (10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49) or
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation / Emission / Laser:
350 nm / 660 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It consists of CD3 gamma, delta, epsilon, and zeta subunits, encoded on chromosome 11. CD3 is expressed on most T lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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