Thermo Fisher Scientific Human AMPK alpha-1/beta-2/gamma-1, His Tag Recombinant Protein
Human AMPK α1/β2/γ1 recombinant protein with His-tag, expressed in baculovirus system. Suitable for kinase assays and LanthaScreen™ binding studies. Purified, liquid form with optimized storage buffer. Stable at -80°C for up to 6 months.
- 카탈로그번호
- PV624x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Kinase Assay (KA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Baculovirus |
| Amino Acid Sequence | 10-end |
| Tag | His-tag |
| Molecular Weight | 63.6–36–40 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM sodium phosphate (pH 7), 150 mM imidazole, 25% glycerol, 300 mM NaCl, 0.1 mM PMSF, 0.2 mM DTT |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
For maximum recovery, spin prior to use. Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use material in original packaging until exhausted. For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL). Do not store kinase diluted. When properly stored at -80°C, product is guaranteed for 6 months from date of purchase.
Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Target Information
AMPK (A1/B2/G1) is a member of the AMPK family, heterotrimeric proteins consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. AMPKs are key energy-sensing enzymes that monitor cellular energy status, particularly under stress. They regulate fatty acid and cholesterol synthesis by controlling rate-limiting enzymes such as acetyl-CoA carboxylase and HMG-CoA reductase. The beta subunit may positively regulate AMPK activity and is highly expressed in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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