Thermo Fisher Scientific ATG4B Polyclonal Antibody
Rabbit polyclonal antibody targeting human ATG4B protein. Validated for WB and IHC applications. Lyophilized form for flexible storage. Suitable for autophagy research. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 20–80 of human ATG4B conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1089651 |
Product Specific Information
Reconstitute with 150 µL of distilled water.
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. ATG4B is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to reveal a C-terminal glycine. Exposure of this glycine is essential for ATG8 protein conjugation to phosphatidylethanolamine (PE) and membrane insertion, which are necessary for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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