Thermo Fisher Scientific Phospho-Cofilin (Ser3) Polyclonal Antibody
Phospho-Cofilin (Ser3) 폴리클로날 항체로 인간, 마우스, 랫에서 반응하며 Western blot, IHC, ICC, ELISA에 적합. 액상 형태로 PBS 버퍼에 보관되며 친화성 크로마토그래피로 정제됨. 세포골격 및 세포 형태 조절 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-Cofilin (Ser3) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S3 of human CFL1 (NP_005498.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.06 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914839 |
Product Specific Information
Positive test controls include NIH/3T3.
The target is usually found in the following locations: Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane.
Immunogen sequence: MASGV
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity.
Regulates actin cytoskeleton dynamics and is essential for normal mitosis and cytokinesis.
Plays a role in cell morphology regulation and cytoskeletal organization.
Required for up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing efficiency in chemokine uptake and degradation.
Also required for neural tube morphogenesis and neural crest cell migration.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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